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The European Arabidopsis Stock Centre

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NASC ID: N849597

Name: WiscDsLox245B05

ABRC stock number: CS849597

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox245B05.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G43810.1 and AT1G43830.1-P355 (chr 1 pos 16584379 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796461.

Wiscseq_DsLox245B05.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G43546.1 (chr 3 pos 15445092 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796462.

Wiscseq_DsLox245B05.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G24430.1 (at chr 3 pos 8869470 (C/8869470-8869564) on the TAIR10). The sequences' 1-95 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 5, C 7 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452990.

Wiscseq_DsLox245B05.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G36740.1 (chr 5 pos 14462709 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796464.

Wiscseq_DsLox245B05.5 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G19900.1 (chr 5 pos 6728907 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796463.

Donation Date: 2004-12-01

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox245B05

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code: